5DEV
High resolution structure of CCG DNA repeats at 1.71 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.228, 38.228, 54.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.030 - 1.710 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 3.753 |
Phasing software | PHENIX (1.8.4-1496) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.770 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.035 | 0.474 |
Number of reflections | 4687 | |
<I/σ(I)> | 67.391 | 4 |
Completeness [%] | 99.6 | 100 |
Redundancy | 13.1 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 50 mM Sodium cacodylate, 1 mM magnesium chloride, 3% 2-methylpentane-2,4-diol, 0.5 mM Cobalt(II) chloride |