5DET
X-ray structure of human RBPMS in complex with the RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.800, 90.232, 34.160 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 19.867 - 1.950 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cyj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.790 |
| Rmerge | 0.120 | 0.775 |
| Number of reflections | 17527 | |
| <I/σ(I)> | 10 | 1.8 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 6.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris-HCl pH 6.5, 28% (w/v) PEG 2000 MME |
