5DEM
Structure of Pseudomonas aeruginosa LpxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-01-04 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.031, 82.176, 220.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.260 - 1.810 |
| R-factor | 0.18578 |
| Rwork | 0.184 |
| R-free | 0.21135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.397 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 110.260 |
| High resolution limit [Å] | 1.809 |
| Number of reflections | 130011 |
| <I/σ(I)> | 38.54 |
| Completeness [%] | 98.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M calcium acetate, 0.1M imidazole pH8.0, 20% PEG 1000 |
