5DEA
Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, cesium bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-03 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.4938 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.570, 129.870, 36.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.867 - 2.797 |
| R-factor | 0.2027 |
| Rwork | 0.202 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5de8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.870 |
| High resolution limit [Å] | 2.797 | 2.797 |
| Rmerge | 0.074 | 0.509 |
| Number of reflections | 12553 | |
| <I/σ(I)> | 15.3 | 4.4 |
| Completeness [%] | 97.3 | 69.5 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate pH 5.6, 0.1 M ammonium acetate, 0.1 M cesium chloride, 18% PEG 4000 |
