5DEA

Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, cesium bound form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-03
Detector	ADSC QUANTUM 1
Wavelength(s)	1.4938
Spacegroup name	P 21 21 2
Unit cell lengths	56.570, 129.870, 36.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.867 - 2.797
R-factor	0.2027
Rwork	0.202
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5de8
RMSD bond length	0.004
RMSD bond angle	1.287
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.870
High resolution limit [Å]	2.797	2.797
Rmerge	0.074	0.509
Number of reflections	12553
<I/σ(I)>	15.3	4.4
Completeness [%]	97.3	69.5
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M sodium citrate pH 5.6, 0.1 M ammonium acetate, 0.1 M cesium chloride, 18% PEG 4000