5DDS
Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.078, 84.210, 88.949 |
| Unit cell angles | 106.71, 109.16, 95.03 |
Refinement procedure
| Resolution | 62.020 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.578 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.920 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.136 | 0.547 |
| Number of reflections | 61697 | |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 92.8 | 89.5 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium acetate, 0.1M TRIS pH 8.5, 32% PEG3350, 2% glycerol |
