5DDP
L-glutamine riboswitch bound with L-glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.648, 86.205, 60.713 |
| Unit cell angles | 90.00, 100.04, 90.00 |
Refinement procedure
| Resolution | 38.989 - 2.302 |
| R-factor | 0.1929 |
| Rwork | 0.190 |
| R-free | 0.24300 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 27308 | |
| <I/σ(I)> | 18.1 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.1 M HEPES-sodium, pH 7.0, 40% (v/v) 2-methyl-2,4-pentanediol |
