5DDM

Human DNA polymerase lambda- Apoenzyme and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	95
Detector technology	CCD
Collection date	2013-10-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	83.693, 83.693, 396.326
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.983 - 2.802
R-factor	0.205
Rwork	0.201
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.091	0.034	0.504
Rmeas	0.097	0.037	0.539
Rpim	0.033	0.013	0.186
Total number of observations	180828
Number of reflections	21271
<I/σ(I)>	6
Completeness [%]	99.2	96.9	100
Redundancy	8.5	7.7	8.1
CC(1/2)		0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate