5DDJ

Crystal structure of recombinant foot-and-mouth-disease virus O1M-S2093Y empty capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	298
Detector technology	PIXEL
Collection date	2012-05-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	I 2 3
Unit cell lengths	344.080, 344.080, 344.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.664 - 3.500
Rwork	0.361
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fod
RMSD bond length	0.011
RMSD bond angle	1.810
Data scaling software	HKL-2000
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.427	0.140	0.938
Total number of observations	67562
Number of reflections	45271
<I/σ(I)>	1.7
Completeness [%]	53.2	55.1	47.9
Redundancy	1.5	1.6	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	1.5 M ammonium sulphate, 100 mM bis-Tris Propane, pH 7.0