5DD6
Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-06-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.557, 66.128, 73.346 |
| Unit cell angles | 116.94, 104.73, 92.90 |
Refinement procedure
| Resolution | 29.391 - 1.699 |
| R-factor | 0.1913 |
| Rwork | 0.191 |
| R-free | 0.22430 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 87986 |
| <I/σ(I)> | 18.8 |
| Completeness [%] | 94.7 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium/sodium tartrate, 20% PEG 3350 |
