5DD3

Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2014-05-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	43.252, 48.663, 54.787
Unit cell angles	90.70, 104.93, 96.62

Refinement procedure

Resolution	22.878 - 1.797
R-factor	0.202
Rwork	0.199
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dd1
RMSD bond length	0.007
RMSD bond angle	1.132
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	37018
<I/σ(I)>	27.4
Completeness [%]	95.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaI, 20% PEG 3350
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaI, 20% PEG 3350