5DCQ

Crystal structure of bacterial adhesin, FNE from Streptococcus equi spp. equi.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	153
Detector technology	PIXEL
Collection date	2012-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	P 61
Unit cell lengths	113.688, 113.688, 152.623
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.830
R-factor	0.1688
Rwork	0.167
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi9 3ltj
RMSD bond length	0.013
RMSD bond angle	1.501
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.830	1.830
Number of reflections	91861
<I/σ(I)>	14.2	1.9
Completeness [%]	97.8	97.7
Redundancy	2.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	magnesium formate, PEG 3350