5DCE
Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase regulated (Tryptophan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-07-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.432, 138.042, 75.868 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 75.370 - 2.230 |
| R-factor | 0.19449 |
| Rwork | 0.192 |
| R-free | 0.23784 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.262 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.370 | 2.280 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.086 | 1.100 |
| Number of reflections | 72956 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 296 | 0.1M Tris HCl (pH 7.3), 0.2 M trimethyl-amino-N-oxide (TMAO), 0.4 mM MnSO4 and PEG 2000MME. |
