5DBU

Crystal structure of 2-deoxyribose-5-phosphate aldolase (1-220) from Streptococcus suis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	192.816, 107.134, 180.966
Unit cell angles	90.00, 110.50, 90.00

Refinement procedure

Resolution	38.311 - 2.797
R-factor	0.2253
Rwork	0.223
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ng3
RMSD bond length	0.008
RMSD bond angle	1.447
Phasing software	MOLREP (11.0)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.114	0.464
Number of reflections	84890
<I/σ(I)>	31.729	3.73
Completeness [%]	99.7	98.8
Redundancy	6.4	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100mM Na citrate, 200mM Na phosphate monobasic monohydrate, 20% Peg 3350