5DBT
Crystal structure of C-terminal truncated 2-deoxyribose-5-phosphate aldolase (1-201) from Streptococcus suis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00150 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.993, 163.662, 169.448 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 45.294 - 2.811 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ng3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.921 |
| Phasing software | MOLREP (11.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.118 | 0.448 |
| Number of reflections | 69858 | |
| <I/σ(I)> | 15.067 | 3.706 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M sodium formate, 20%(w/v) PEG 3350, 40% pentaerythritol ethoxylate (3/4 EO/OH) |
