5DBK
apo form of the quorum sensor NprR from B. thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.789, 105.337, 177.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.929 - 3.241 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.24470 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.440 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.120 | 0.670 |
| Number of reflections | 20499 | |
| <I/σ(I)> | 10.03 | 2.18 |
| Completeness [%] | 98.4 | 95.8 |
| Redundancy | 3.46 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 291 | NA CITRATE 1.0 M, HEPES 100 MM, PH 7.6, VAPOR DIFFUSION, TEMPERATURE 291K |
