5DAY

The structure of NAP1-Related Protein(NRP1) in Arabidopsis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97900, 0.97946, 0.97930, 0.95365
Spacegroup name	P 21 21 21
Unit cell lengths	60.542, 60.812, 135.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.218 - 2.329
R-factor	0.2336
Rwork	0.230
R-free	0.26880
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.833
Data reduction software	HKL-2000
Data scaling software	Coot
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2152)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.329	2.330
Number of reflections	21817
<I/σ(I)>	43.7
Completeness [%]	99.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	289.15	25% PEG 400, 0.1 M HEPES pH 7.6, 0.2 M Calcium chloride dihydrate