5DA4
Structure of a nanobody recognizing the fumarate transporter SLC26Dg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 139.511, 139.511, 196.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.996 - 2.400 |
| R-factor | 0.2333 |
| Rwork | 0.233 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4WGV |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 1.357 |
| Number of reflections | 76545 | |
| <I/σ(I)> | 23.5 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 14.9 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M Hepes pH 7.5, 200 mM CaCl2, 28% PEG400 |
