5D9X
Dehydroascorbate reductase complexed with GSH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.890, 47.600, 51.790 |
| Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
| Resolution | 39.476 - 1.680 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.530 |
| High resolution limit [Å] | 1.680 |
| Number of reflections | 24925 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 98.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM Bis-Tris HCl, pH 6.6, 28%(w/v) PEG3350, 10mM CaCl2 |
