5D9W
Dehydroascorbate reductase (OsDHAR) complexed with ASA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.848, 47.279, 50.992 |
Unit cell angles | 90.00, 108.23, 90.00 |
Refinement procedure
Resolution | 28.611 - 1.690 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.908 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.690 |
Number of reflections | 23333 |
<I/σ(I)> | 53.8 |
Completeness [%] | 98.2 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM Bis-Tris HCl, pH 6.6, 28%(w/v) PEG3350, 10mM CaCl2 |