5D9S
Structure of HLA-A2:01 with the 11-mer peptide F11V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.330, 80.238, 56.855 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 26.530 - 1.870 |
| R-factor | 0.19095 |
| Rwork | 0.190 |
| R-free | 0.22305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mre |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.144 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 36209 | |
| <I/σ(I)> | 25.6 | 2.7 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30% PEG 5000 MME, 0.1M Tris-HCl pH 8.0, 0.2M lithium sulfate |
