5D96

Oxidoreductase Fragment of Mouse QSOX1 in Complex with a FAb Fragment from an Antibody Targeting Mouse and Human QSOX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-06-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	78.799, 204.770, 44.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.424 - 2.300
R-factor	0.1704
Rwork	0.167
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d8i 4ij3
RMSD bond length	0.004
RMSD bond angle	1.014
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.084	0.418
Number of reflections	62461
<I/σ(I)>	8.3	2.2
Completeness [%]	99.7	100
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	50 mM CaCl2, 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer, pH 6, 22.5% w/v PEG 6 kD