5D90

Crystal structure of HiNmlR, a MerR family regulator lacking the sensor domain, bound to promoter DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.972
Spacegroup name	I 1 2 1
Unit cell lengths	107.633, 38.967, 145.549
Unit cell angles	90.00, 97.16, 90.00

Refinement procedure

Resolution	19.800 - 2.300
R-factor	0.2247
Rwork	0.221
R-free	0.26970
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.894
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.800
High resolution limit [Å]	2.300
Number of reflections	27154
<I/σ(I)>	6.5
Completeness [%]	99.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Bis-Tris pH 6.5, 25 % PEG 3350