5D8V
Ultra-high resolution structure of high-potential iron-sulfur protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.45086 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.478, 58.905, 23.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.480 |
| Rwork | 0.072 |
| R-free | 0.07800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a39 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | MOPRO (11.10) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.500 |
| High resolution limit [Å] | 0.480 | 0.480 |
| Rmerge | 0.056 | 0.339 |
| Number of reflections | 301119 | |
| <I/σ(I)> | 61.1 | 2.7 |
| Completeness [%] | 96.3 | 89 |
| Redundancy | 5.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1.6 M ammonium sulfate, 0.1 M sodium-citrate, 5 mM DTT |
