5D8L
Human HSF2 DNA Binding Domain in complex with 3-site HSE DNA at 2.1 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.174, 47.140, 109.587 |
| Unit cell angles | 82.82, 90.03, 64.75 |
Refinement procedure
| Resolution | 24.410 - 2.069 |
| R-factor | 0.2199 |
| Rwork | 0.217 |
| R-free | 0.27300 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1675) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.410 | 2.120 |
| High resolution limit [Å] | 2.069 | 2.069 |
| Number of reflections | 38940 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 88.5 | 85 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 7 mg/ml protein:DNA complexes were mixed at a ratio of 1:1.2 protein:DNA in 25 mM HEPES pH 7.5 and 150 mM NaCl and crystallized against 170 mM ammonium acetate, 85 mM sodium acetate pH 4.6, 25.5% PEG 4000, and 15% Glycerol. Crystals grew in 3-4 days |
