5D8K
Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-08-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.883, 39.631, 39.393 |
| Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
| Resolution | 21.210 - 1.728 |
| R-factor | 0.1531 |
| Rwork | 0.148 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.396 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1675) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.210 | 1.760 |
| High resolution limit [Å] | 1.728 | 1.730 |
| Number of reflections | 13023 | |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 93.9 | 89.7 |
| Redundancy | 5.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295 | 7 mg/ml protein:DNA complexes were mixed at a ratio of 1:1.2 protein:DNA in 25 mM HEPES pH 7.5 and 150 mM NaCl and crystallized against 100 mM NaCl, 100 mM BICINE pH 9, and 30% PEG 550 MME. Crystals grew overnight at room temperature |
