5D8I
Engineering the Species-Specificity of an Inhibitory Antibody Targeting a Modulator of Tumor Stroma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.480, 116.375, 50.023 |
| Unit cell angles | 90.00, 103.09, 90.00 |
Refinement procedure
| Resolution | 28.513 - 2.050 |
| R-factor | 0.2045 |
| Rwork | 0.201 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q6o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.275 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.063 | 0.318 |
| Number of reflections | 28528 | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 95.8 | 79.2 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 5% w/v dimethyl sulfoxide, 0.1 M sodium acetate, pH 4.6, 7% w/v PEG monomethyl ether 2 kD |
