5D8F
crystal structure of SSB and ssDNA complex from homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 83.821, 83.821, 159.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.910 - 2.350 |
| R-factor | 0.2407 |
| Rwork | 0.238 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d8e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.063 | 0.507 |
| Number of reflections | 14419 | |
| <I/σ(I)> | 73 | 9.16 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 19.9 | 21.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MES pH 6.0, 16% Jeffamine M-600 |
