5D8E
crystal structure of SSB from homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.691, 137.691, 83.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.914 - 3.000 |
R-factor | 0.2673 |
Rwork | 0.264 |
R-free | 0.28930 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.084 | 0.430 |
Number of reflections | 18515 | |
<I/σ(I)> | 50.1 | 8.65 |
Completeness [%] | 98.8 | 100 |
Redundancy | 9 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MES pH 6.0, 16% Jeffamine M-600 |