5D8A

Crystal structure of recombinant foot-and-mouth-disease virus A22-H2093F empty capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	298
Detector technology	PIXEL
Collection date	2013-06-24
Detector	PSI PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	I 2 2 2
Unit cell lengths	327.570, 341.290, 363.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
Rwork	0.206
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iv1
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.265	0.126
Total number of observations	498664
Number of reflections	356924
<I/σ(I)>	2.8
Completeness [%]	45.7	46.3	39.8
Redundancy	1.4	1.4	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	4 M ammonium acetate, 100 mM bis-Tris Propane