5D7W
Crystal structure of serralysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.300, 105.400, 151.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.628 - 1.100 |
| R-factor | 0.1759 |
| Rwork | 0.175 |
| R-free | 0.18610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i35 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.019 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.091 | |
| Number of reflections | 273151 | |
| <I/σ(I)> | 10.96 | 2.03 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.01 | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 289 | PEG 8000,Ammonium sulfate,sodium citrate |
