5D7J
Structure of human MR1-5-OP-RU in complex with human MAIT M33.64(Y95alphaF) TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.600, 69.590, 141.470 |
| Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
| Resolution | 35.757 - 1.970 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l4t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 135443 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 93.8 | 77 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | BTP, PEG 3350, NaAc |
