5D79
Structure of BBE-like #28 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91964 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 127.024, 133.132, 138.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.887 - 1.849 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d2h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.890 | 1.915 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Rmerge | 0.122 | 0.558 |
| Number of reflections | 93337 | |
| <I/σ(I)> | 7.22 | |
| Completeness [%] | 93.2 | 91.72 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 293 | 0.1 M HEPES buffer pH 7.0, 30 % v/v Jeffamine ED 2001 |
