5D77
Structure of Mip6 RRM3 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.310, 36.310, 91.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.766 - 1.300 |
| R-factor | 0.1529 |
| Rwork | 0.151 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fey |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.450 | |
| Number of reflections | 15814 | |
| <I/σ(I)> | 25.1 | 3.5 |
| Completeness [%] | 99.4 | 95.9 |
| Redundancy | 11.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 1.6M Sodium Citrate Tribasic dihydrate pH 6.5; Ammonium Nitrate |
