Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.390, 93.150, 115.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.330 |
| R-factor | 0.20498 |
| Rwork | 0.202 |
| R-free | 0.25809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tu7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.618 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.113 | 0.317 |
| Number of reflections | 21281 | |
| <I/σ(I)> | 9.7 | 4.3 |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.2M MAGNESIUM ACETATE, 0.1M SODIUM CACODYLATE, 10% (W/V) PEG 8000; 15% GLYCEROL WAS USED AS CRYOPROTECTANT FOR DATA COLLECTION |
