5D6X

Crystal structure of double tudor domain of human lysine demethylase KDM4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	I 21 3
Unit cell lengths	134.829, 134.829, 134.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.600 - 2.153
R-factor	0.1622
Rwork	0.159
R-free	0.19970
RMSD bond length	0.008
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.600		2.280
High resolution limit [Å]	2.150	6.410	2.150
Rmerge	0.073	0.042	0.448
Rmeas	0.079	0.045	0.484
Total number of observations	306499
Number of reflections	42897	1630	6933
<I/σ(I)>	17.97	40.51	4.38
Completeness [%]	100.0	99.6	99.9
Redundancy	7.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein Solution (30mg/ml KDM4A DTD, 20mM HEPES pH 7.5, 150mM NaCl and 0.5mM TCEP) mixed in a 1:1 ratio with the well solution (1.0M ammonium sulfate, 1% w/v peg3350 and 0.1M Bis-Tris pH5.5) Cryoprotected with additional 20% glycerol