5D6U
X-ray crystal structure of ribonuclease A determined for the real space D/H contrast method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2012-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.340, 38.369, 53.188 |
| Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
| Resolution | 16.861 - 1.520 |
| R-factor | 0.1318 |
| Rwork | 0.131 |
| R-free | 0.15570 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.900 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.058 | 0.136 |
| Number of reflections | 16748 | |
| <I/σ(I)> | 43.3 | 20.8 |
| Completeness [%] | 91.7 | 95.2 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.8 | 293 | 20 mg/mL ribonuclease A, 0.05 M sodium acetate, 50% 2-propanol |
