5D6S
Structure of epoxyqueuosine reductase from Streptococcus thermophilus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 106.120, 106.120, 332.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.580 - 2.650 |
| R-factor | 0.21601 |
| Rwork | 0.214 |
| R-free | 0.25301 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.976 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.580 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.126 | |
| Number of reflections | 60261 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 98.8 | 97.8 |
| Redundancy | 14 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | Anaerobic crystals were obtained by mixing 2 microL protein with 2 microL 0.3 M sodium acetate, 0.1 M Tris/Cl pH7.5, 15% w/v PEG4000 and incubated at room temperature in a 100% N2-atmosphere glove box |
