5D6I
DgkA - CIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.765, 72.765, 199.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.758 - 3.091 |
| R-factor | 0.267 |
| Rwork | 0.266 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ze5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.467 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.760 |
| High resolution limit [Å] | 3.090 |
| Number of reflections | 11778 |
| <I/σ(I)> | 1.4 |
| Completeness [%] | 99.7 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 6-11.5% MPD, 3% 1,4 butanediol, 0.1 M Li nitrate, 0.1 M NaCl, 0.1 M Na citrate pH 5.6. |
