5D6H
Crystal structure of CsuC-CsuA/B chaperone-major subunit pre-assembly complex from Csu biofilm-mediating pili of Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 94.705, 94.705, 187.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.904 - 2.400 |
| R-factor | 0.2237 |
| Rwork | 0.219 |
| R-free | 0.26580 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.762 | 46.762 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.029 | 0.539 | |
| Rmeas | 0.054 | ||
| Rpim | 0.014 | 0.013 | 0.135 |
| Total number of observations | 305639 | 8924 | 46837 |
| Number of reflections | 36640 | ||
| <I/σ(I)> | 37.4 | 81 | 6.3 |
| Completeness [%] | 99.8 | 97.8 | 100 |
| Redundancy | 15.2 | 11.9 | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1 M sodium malonate, 15% PEG 3350 |
