5D5S

Crystal structure of methionine gamma-lyase from Citrobacter freundii, S339A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	I 2 2 2
Unit cell lengths	56.659, 123.090, 128.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.1745
Rwork	0.173
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rfv
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.045
Number of reflections	49574
<I/σ(I)>	23.5	4.7
Completeness [%]	99.4	97.5
Redundancy	7	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	303	5% PEG2000 MME, 50 MM TRIS-HCL, 0.2 MM PLP, 0.25% DTT, PH 8.5,