5D5P
HcgB from Methanococcus maripaludis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.120, 72.540, 70.980 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 43.675 - 1.700 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wb1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 | |
| High resolution limit [Å] | 1.700 | 12.000 | 1.700 |
| Rmerge | 0.045 | 0.018 | 0.730 |
| Rmeas | 0.052 | 0.021 | 0.848 |
| Total number of observations | 272465 | ||
| Number of reflections | 70410 | 213 | 11005 |
| <I/σ(I)> | 18.08 | 62.1 | 2.55 |
| Completeness [%] | 99.6 | 95.9 | 99.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 281 | 0.1 M CAPS (pH 10.5), 0.2 M NaCl, 20 % (w/v) PEG8000 |
