5D52
In meso in situ serial X-ray crystallography structure of insulin at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | I 21 3 |
Unit cell lengths | 79.710, 79.710, 79.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.855 - 1.800 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9ins |
RMSD bond length | 0.012 |
RMSD bond angle | 1.094 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 7975 | |
<I/σ(I)> | 6.96 | 0.88 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.2 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400 |