5D4W
Crystal structure of Hsp104
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.920, 93.170, 144.416 |
| Unit cell angles | 90.00, 119.72, 90.00 |
Refinement procedure
| Resolution | 47.583 - 3.700 |
| R-factor | 0.2386 |
| Rwork | 0.237 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ClpB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2356: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.583 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.192 | 1.624 |
| Rpim | 0.130 | 1.121 |
| Number of reflections | 35560 | |
| <I/σ(I)> | 5.3 | 0.8 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 8.3% penta-erythritol propoxylate and 0.1 M MES-NaOH pH 5.8 |
