5D4O
Structure of CPII, a nitrogen regulatory PII-like protein from Thiomonas intermedia K12, bound to ADP, AMP and bicarbonate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.440, 75.450, 80.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.900 - 1.800 |
| R-factor | 0.2072 |
| Rwork | 0.206 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d4l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.840 | ||
| High resolution limit [Å] | 1.800 | 8.030 | 1.800 |
| Rmerge | 0.050 | 0.024 | 0.563 |
| Rmeas | 0.055 | 0.027 | 0.618 |
| Total number of observations | 191688 | ||
| Number of reflections | 29590 | 392 | 2037 |
| <I/σ(I)> | 19.24 | 50.92 | 2.74 |
| Completeness [%] | 99.4 | 99.7 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.15 M KBr, 30% (w/v) PEG MME 2000 |
