5D3O

Crystal structure of full length human glutaminase C expressed in E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.920
Spacegroup name	P 2 21 21
Unit cell lengths	51.609, 139.371, 179.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.772 - 2.791
R-factor	0.2167
Rwork	0.214
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.790
Rmerge	0.108
Number of reflections	32955
<I/σ(I)>	16.4
Completeness [%]	99.3
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% PEG6000, 1.0M LiCl, 0.1M Tris-HCL, pH8.0