5D39
Transcription factor-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-22 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.395, 94.697, 145.642 |
| Unit cell angles | 79.62, 78.31, 89.58 |
Refinement procedure
| Resolution | 44.120 - 3.200 |
| R-factor | 0.2111 |
| Rwork | 0.210 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y5u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.082 | 0.695 |
| Number of reflections | 57110 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M citrate (pH 5.6), 0.1 M NaCl, 20% isopropyl alcohol and 8% PEG4000 |
