5D2S

Crystal structure of STPR from Bombyx mori in complex with 18-bp DNA containing four repetitive units of ATAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	44.011, 44.011, 237.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.280 - 2.200
R-factor	0.22808
Rwork	0.227
R-free	0.25324
RMSD bond length	0.014
RMSD bond angle	1.848
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	22628
<I/σ(I)>	14.4
Completeness [%]	99.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	18% PEG 2000, 0.1 M sodium citrate pH5.6