5D1X

IsdB NEAT2 bound by D4-30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	90
Detector technology	CCD
Collection date	2014-07-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 41
Unit cell lengths	111.251, 111.251, 105.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 3.210
R-factor	0.27138
Rwork	0.269
R-free	0.30996
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.465
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.790
High resolution limit [Å]	3.210
<I/σ(I)>	9.1
Completeness [%]	99.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		290	15% PEG10k 0.1M Tris pH 8