5D1O

Archaeal ATP-dependent RNA ligase - form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-21
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5414
Spacegroup name	P 1 21 1
Unit cell lengths	50.250, 114.870, 91.290
Unit cell angles	90.00, 104.42, 90.00

Refinement procedure

Resolution	27.713 - 2.648
R-factor	0.209
Rwork	0.206
R-free	0.26530
RMSD bond length	0.050
RMSD bond angle	1.404
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Number of reflections	32926
<I/σ(I)>	4.83
Completeness [%]	94.4
Redundancy	2.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris-HCl (pH 8.5), 0.2 M Lithium Sulfate and 40 % PEG400