5D1C
Crystal structure of D233G-Y306F HDAC8 in complex with a tetrapeptide substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.031, 97.936, 104.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.353 - 1.422 |
R-factor | 0.1474 |
Rwork | 0.146 |
R-free | 0.16700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ewf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.272 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1833) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.400 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.072 | 1.112 |
Number of reflections | 159962 | |
<I/σ(I)> | 28.5 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.5 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 100 mM Tris (pH 8.0), 10% (w/v) PEG 3350, 4 mM TCEP |